Two-photon multi-color imaging of memory circuits in the fixed and transparent brain

We are developing new optogenetic probes that allow labeling and optical manipulation of dendritic spines underlying specific learning and memory tasks in mice. Subsequently, we will label axons involved in memory, and thereby identify memory circuits from the memory synapses. We will also make use of various gene induction protocols to simultaneously label multiple memory circuits to assist physiological and pathological research on primates, which are more complex and exhibit larger individual variability.