(E) Development of a high-performance, wide and contiguous field-of-view two-photon microscope

The brain functions are likely based on the coordinated activity of multiple brain regions mediated by long/middle-range connections. To understand the causal relationship between the coordinated activity and brain functions, we attempt to develop a wide and contiguous field-of-view two-photon microscope that allows optogenetic manipulation and multi-color imaging. We use various calcium sensors and big data analysis methods by collaborating with other research groups. We record the neural activity of mice and marmosets during perception, cognition, and social behavioral tasks, and aim to elucidate the principle of network dynamics underlying the brain functions.