To identify descending pathways with latent potential for functional recovery, we systematically map brain-spinal functions in stroke patients and stroke model macaques using MRI imaging and neurophysiological techniques. By implementing inhibitory Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) within the descending pathways of stroke model macaques, specifically targeting those pathways corresponding to latent recovery potential identified in human stroke patients, we aim to identify descending pathways engaged in recovery processes. Moreover, to augment functional recovery, we activate these identified pathways using excitatory DREADDs and assess whether this targeted activation enhances recovery outcomes.