Low molecular metabolites, such as sugars and nucleic acids, are essential molecules transported inside and outside cells, playing crucial roles in signal transduction and regulating energy states. The significance of metabolic changes in the brain has been highlighted in neuropsychiatric disorders, yet the mechanisms and implications remain poorly understood. Therefore, this study aims to establish a single-cell level imaging metabolome analysis technique and apply it to a mouse stress model, aiming to elucidate the nature and significance of metabolic changes that underlie stress-induced alterations in brain function.