Astrocytes actively contribute to the formation and functional regulation of neural circuits by adhering to neuronal synapses through fine processes, forming tripartite synapses. Recent genetic and chemogenetic analyses have suggested that astrocytic cellular activity and dysfunction are closely related to many psychiatric disorders. However, despite the involvement of unique intracellular signaling pathways in astrocytic cellular activity, much remains unknown about the molecular dynamics and mechanisms underlying these processes. This research project aims to develop an innovative spatial proteome sensor that combines advanced cellular activity imaging and proteomic analysis, surpassing conventional methods. Through this approach, we aim to rapidly visualize activity changes in astrocytes associated with the onset of psychiatric disorders and elucidate the molecular mechanisms underlying these changes in vivo.