By integrating and advancing our independently developed, pioneering optical microscopy technologies, we aim to establish an imaging platform centered on live super-resolution imaging of deep brain regions. This platform will play a crucial role in combating neurodegenerative diseases and facilitate technical collaborations within and beyond the program. In particular, we will utilize the novel technique of PSF (Point Spread Function) engineering to build a seamless imaging system capable of zooming in and out across spatial and temporal scales—from "volume" observation of the live brain in model animals to video-rate imaging or super-resolution imaging, respectively. This system will enable visualization of the higher-order structural information of biomolecules in vivo.