Development of an innovative embryo manipulating system for the generation of genetically modified marmosets

This research project will develop reliable low-priced technology and the human resources to create genetically modified marmoset. We hope to improve the access to marmosets for scientific experimentation. The project comprises three aims. The first is develop a stable method for generating mature oocyte and fertilized eggs. The second is generate model animals for brain diseases via genome editing using fertilized eggs from xenotransplanted ovaries. The third is establish naïve ES cells and generate genetically modified marmosets using the blastocyst complementation method.