Production of knockout marmoset using spermatogenic cells

Knockout animals can be produced using germline stem (GS) cells, cultured spermatogonia with enriched spermatogonial stem cell (SSC) activity. However, little is known about SSCs in primates. In this study, we will develop an SSC enrichment protocol and aim to establish GS cells from marmoset testis by analyzing the gene expression pattern in SSCs. We will also try to carry out gene editing using spermatogenic cells in vivo.